Glen Research Product Information

Catalog Number: 10-5944-xx
Description: BBQ-650®-dT-CE Phosphoramidite

5'-Dimethoxytrityloxy-5-[6-(9-[4-nitro-2',5'-dimethoxyazobenz-4'-yl diazo]-julolidin-8-oxy)-hexylamidoethyl-3-acrylimido]-2'-deoxyUridine -3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Formula: C76H89N12O15
M.W.: 1441.57 
F.W.: 1000.95 

Diluent: Anhydrous Acetonitrile/Dichloromethane 1:3 (v/v)

Coupling: A coupling time of 15 minutes is recommended.

Deprotection: Complete the deprotection using the protocol required by the nucleobases. Compatible with standard deprotection conditions in Ammonium Hydroxide and room temperature AMA (Ammonium hydroxide/40% Methylamine 1:1 v/v) for 2 hours.

Storage: Freezer storage, -10 to -30°C, dry. Light sensitive material.

Stability in Solution: 1-2 days

Please Note: BlackBerry® Quencher technology: US Patent 7,879,986. The purchase of BlackBerry® Quencher reagents includes a limited license to use these reagents exclusively for research and development purposes. They may not be used for clinical or diagnostic purposes and they may not be re-sold, distributed, or re-packaged without prior agreement and consent of Berry & Associates, Inc. Subsequent sale of products that are derived from BlackBerry® Quencher reagents is permitted so long as the following written disclaimer is included in written and electronic catalogs, in commercial advertisement, and in packages with containers of such derivative products: "BlackBerry is a trademark of Berry & Associates, Inc. Products derived from BlackBerry® Quencher reagents are sold exclusively for research and development use by the purchaser. They may not be used for clinical or diagnostic purposes without prior agreement and consent of Berry & Associates, Inc."

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


10-5944-95
50µm
280.00
10-5944-90
100µm
545.00
10-5944-02
0.25g
925.00

Related Document(s)

  

Literature Highlight(s)

FREQUENTLY ASKED TECHNICAL QUESTION

QUESTION: What are the relative extinction coefficients of 5'-Fluorescein, Hex and Tet etc.. at 260 nm and their Lambda max?

RESPONSE:Please see http://www.glenresearch.com/Technical/Extinctions.html

REFERENCE(S):
Oligonucleotide Properties Calculator; http://www.basic.northwestern.edu/biotools/oligocalc.html


QUESTION: Dye Quencher Plot

RESPONSE:Dye Quencher Plot


DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-5944-020.25grams.25grams1.7344.3326.616.6312.098.872.22
10-5944-90100µmoles.144grams120127.55.4541
10-5944-9550µmoles.072grams.53.3321.25.91.67.17
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-5944-020.25grams.25grams2.59.0745.428.3820.642.84
10-5944-90100µmoles.144grams1.5.0723.614.7510.731.48
10-5944-9550µmoles.072grams.75.078.65.383.91.54

 

 

VIEW PRINTABLE VERSION

 

 


  

07/20/2018

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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