Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: Complete the deprotection using the protocol required by the nucleobases. Compatible with standard deprotection conditions in Ammonium Hydroxide and room temperature AMA (Ammonium hydroxide/40% Methylamine 1:1 v/v) for 2 hours.
Storage: Freezer storage, -10 to -30°C, dry. Light sensitive material.
Stability in Solution: 1-2 days
Please Note: BlackBerry® Quencher technology: US Patent 7,879,986. The purchase of BlackBerry® Quencher reagents includes a limited license to use these reagents exclusively for research and development purposes. They may not be used for clinical or diagnostic purposes and they may not be re-sold, distributed, or re-packaged without prior agreement and consent of Berry & Associates, Inc. Subsequent sale of products that are derived from BlackBerry® Quencher reagents is permitted so long as the following written disclaimer is included in written and electronic catalogs, in commercial advertisement, and in packages with containers of such derivative products: "BlackBerry is a trademark of Berry & Associates, Inc. Products derived from BlackBerry® Quencher reagents are sold exclusively for research and development use by the purchaser. They may not be used for clinical or diagnostic purposes without prior agreement and consent of Berry & Associates, Inc."
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.