Glen Research Product Information

Catalog Number: 10-5920-xx
Description: Redmond Red® Phosphoramidite

3-(3-oxo-7-pivaloylphenoxazin-2-yl)-propanamido-1-(2-O-dimethoxytrityloxymethyl)- pyrrolidin-4-yl-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite

Formula: C55H63N4O10
M.W.: 971.09 
F.W.: 445.34 

Diluent: Anhydrous Acetonitrile

Coupling: Monomers that allow for UltraMILD deprotection must be used. (Catalog Numbers: dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx.). To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx)

Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature.

Storage: Freezer storage, -10 to -30°C, dry

Please Note: This product is for research purposes only, and may not be used for commercial, clinical, diagnostic or any other use. The Product is subject to proprietary rights of ELITechGroup and is made and sold under license from ELITechGroup. There is no implied license for commercial use with respect to this Product and a license must be obtained directly from ELITechGroup. with respect to any proposed commercial use of this Product. "Commercial use" includes but is not limited to the sale, lease, license or other transfer of the Product or any material derived or produced from it, the sale, lease, license or other grant of rights to use the Product or any material derived or produced from it, or the use of the Product to perform services for a fee for third parties (including contract research). AquaPhluor®, Yakima Yellow®, Redmond Red® and Eclipse® are registered trademarks of ELITechGroup.

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


10-5920-95
50µm
220.00
10-5920-90
100µm
420.00
10-5920-02
0.25g
1045.00

Related Document(s)

  

Literature Highlight(s)

FREQUENTLY ASKED TECHNICAL QUESTION

QUESTION: What are the relative extinction coefficients of 5'-Fluorescein, Hex and Tet etc.. at 260 nm and their Lambda max?

RESPONSE:Please see http://www.glenresearch.com/Technical/Extinctions.html

REFERENCE(S):
Oligonucleotide Properties Calculator; http://www.basic.northwestern.edu/biotools/oligocalc.html


QUESTION: Does AMA or methylamine cause any degradation to fluorescein or fluorescein-type dyes such as FAM or FITC?

RESPONSE:Response: While AMA (Ammonium hydroxide/40% Methylamine 1:1 v/v) is considered compatible with fluorescein, the use of methylamine when deprotecting a Fluorescein-labeled oligo does lead to a small amount of degradation, which is characterized by a the appearance of a late-eluting peak by RP HPLC that shows no visible fluorescein absorbance. With standard deprotection conditions (AMA 10 minutes at 65 C) the amount of degradation is approximately 5%


DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-5920-020.25grams.25grams2.5772.3343.427.1319.7314.473.62
10-5920-90100µmoles.097grams120127.55.4541
10-5920-9550µmoles.049grams.53.3321.25.91.67.17
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-5920-020.25grams.25grams3.84.0770.444324.4
10-5920-90100µmoles.097grams1.5.0723.614.7510.731.48
10-5920-9550µmoles.049grams.75.078.65.383.91.54

 

 

VIEW PRINTABLE VERSION

 

 


  

07/20/2018

 

 

 

 

 

 

 

 

 

 

 

 

 

 


Contact to place an order.
INTERNATIONAL USERS: Contact your distributor or order from USA
Contact to access more than 50 years of oligo expertise for help in designing oligos for your unique application.
Glen Research Corporation, Copyright © 2018