Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: With the oligonucleotide still attached to the support, treat with 1 M DBU in anhydrous ACN for 2-3 minutes, followed by 17 hours using fresh 1 M DBU at room temperature. Rinse support with ACN and then deprotect as required by nucleobases. Note, the dX nucleoside is susceptible to depurination though short exposure to acid is tolerated - e.g. Glen-Pak purification.
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.