Glen Research Product Information

Catalog Number: 10-1537-xx
Description: dX-CE Phosphoramidite

5'-(4,4'-Dimethoxytrityl)-2,6-O-bis(2-(4-nitrophenyl)ethyl)-2'-deoxyXanthosine, 3’-[(2-cyanoethyl)-N,N-diisopropyl)]-phosphoramidite

Formula: C56H61N8O12
M.W.: 1069.10 
F.W.: 330.19 

Diluent: Anhydrous Acetonitrile

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.

Deprotection: With the oligonucleotide still attached to the support, treat with 1 M DBU in anhydrous ACN for 2-3 minutes, followed by 17 hours using fresh 1 M DBU at room temperature. Rinse support with ACN and then deprotect as required by nucleobases. Note, the dX nucleoside is susceptible to depurination though short exposure to acid is tolerated - e.g. Glen-Pak purification.

Storage: Freezer storage, -10 to -30°C, dry

Stability in Solution: 1-2 days

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


10-1537-95
50µm
150.00
10-1537-90
100µm
280.00
10-1537-02
0.25g
625.00

Related Document(s)

  

Literature Highlight(s)

DILUTION/COUPLING DATA

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-1537-020.25grams.25grams2.3464.6738.824.2517.6412.933.23
10-1537-90100µmoles.107grams120127.55.4541
10-1537-9550µmoles.053grams.53.3321.25.91.67.17
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-1537-020.25grams.25grams3.49.0763.439.6328.823.96
10-1537-90100µmoles.107grams1.5.0723.614.7510.731.48
10-1537-9550µmoles.053grams.75.078.65.383.91.54

 

 

VIEW PRINTABLE VERSION

 

 


  

07/20/2018

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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