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Catalog Number: 20-2015-xx
(20-2115-xx, 20-2215-xx, 20-2515-xx)
Description: Ac-dC-CPG 1000
| 5'-Dimethoxytrityl-N-Acetyl-2'-deoxyCytidine, 3'-succinoyl-long chain alkylamino-CPG 1000 | ||
| F.W.: 289.18 | ||
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: Deprotect using the protocol required by the nucleobases. |
| Storage: Controlled room temperature or lower, dry |
| Stability in Solution: Not Applicable |
| Catalog Information | Material Safety Data Sheet |
Literature Highlights
| Glen Report 6.2: ULTRAFAST DNA SYNTHESIS - 10 MINUTE DEPROTECTION! |
| Glen Report 7.1: ULTRAFAST CHEMISTRY |
Frequently Asked Technical Question
QUESTION: What is the procedure for deprotecting with AMA?
RESPONSE:The AMA reagent is a 50:50 mixture of aqueous Ammonium
hydroxide and aqueous MethylAmine. With AMA the cleavage of the
oligonucleotide from the support is accomplished in 5 minutes at room
temperature. The deprotection step is carried out at 65°C for a
further 5 minutes. Deprotection can also be carried out at lower
temperatures as shown in Table 1. In all cases, no base modification
has been observed.
TABLE 1: DEPROTECTION WITH AMA
| Time | Temperature |
| 5 min | 65°C |
| 10 min | 55°C |
| 30 min | 37°C |
| 90 min | 25°C |
08/15/2012 | http://www.glenres.com/ProductFiles/20-2015.html