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Catalog Number: 10-3730-xx
Description: 2'-Se-Me-U-CE Phosphoramidite
THIS ITEM HAS BEEN DISCONTINUED
| 5'-Dimethoxytrityl-deoxyUridine,2'-methylseleno-3'-[(2-cyanoethyl)- (N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C40H49N4O8PSe | M.W.: 823.78 | F.W.: 383.15 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 10 minute coupling time recommended. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 2-3 days |
| THIS PRODUCT HAS BEEN DISCONTINUED | Material Safety Data Sheet |
Literature Highlights
| Glen Report 16.1: Minor Base and Related Novel Phosphoramidites |
| Glen Report 19.1: Selenium Derivatization of Nucleic Acids for X-ray Crystal Structure Determination |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-3730-02 | 0.25grams | .25grams | 3.03 | 87.67 | 52.6 | 32.88 | 23.91 | 17.53 | 4.38 |
| 10-3730-90 | 100µmoles | .082grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-3730-95 | 50µmoles | .041grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-3730-02 | 0.25grams | .25grams | 4.53 | .07 | 84.2 | 52.63 | 38.27 | 5.26 | |
| 10-3730-90 | 100µmoles | .082grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-3730-95 | 50µmoles | .041grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-3730-02 | 0.25grams | .25grams | 4.53 | .07 | 85.8 | 53.63 | 39 | ||
| 10-3730-90 | 100µmoles | .082grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-3730-95 | 50µmoles | .041grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
08/15/2012 | http://www.glenres.com/ProductFiles/10-3730.html