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Catalog Number: 10-3034-xx
Description: U-TOM-CE Phosphoramidite
| 5'-Dimethoxytrityl-Uridine,2'-O-triisopropylsilyloxymethyl- 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C49H69N4O10PSi | M.W.: 933.17 | F.W.: 306.17 |
Diluent: Anhydrous Acetonitrile |
| Coupling: See Technical Bulletin |
| Deprotection: See Technical Bulletin for details (Technical Bulletin). |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 24 hours |
| Please Note: TOM-RNA Phosphoramidites are supplied under agreement with QIAGEN. RNA synthesis using the TOM-Protecting-Group is covered by US Patent No. 5,986,084. |
| Catalog Information | Material Safety Data Sheet |
Literature Highlights
| Glen Report 11.2: TOM-PROTECTING-GROUP™ - A MAJOR IMPROVEMENT IN RNA SYNTHESIS |
| Glen Report 11.2: TOM-PROTECTING-GROUP™ - A MAJOR IMPROVEMENT IN RNA SYNTHESIS |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-3034-02 | 0.25grams | .25grams | 2.68 | 76 | 45.6 | 28.5 | 20.73 | 15.2 | 3.8 |
| 10-3034-05 | 0.5grams | .5grams | 5.36 | 165.33 | 99.2 | 62 | 45.09 | 33.07 | 8.27 |
| 10-3034-10 | 1.0gram | 1grams | 10.72 | 344 | 206.4 | 129 | 93.82 | 68.8 | 17.2 |
| 10-3034-2S | 20grams | 20grams | 214.32 | 7130.67 | 4278.4 | 2674 | 1944.73 | 1426.13 | 356.53 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-3034-02 | 0.25grams | .25grams | 4 | .07 | N/A | N/A | 15.33 | N/A | |
| 10-3034-05 | 0.5grams | .5grams | 8 | .07 | N/A | N/A | 32 | N/A | |
| 10-3034-10 | 1.0gram | 1grams | 15.99 | .07 | N/A | N/A | 65.29 | N/A | |
| 10-3034-2S | 20grams | 20grams | 319.89 | .07 | N/A | N/A | 1331.54 | N/A | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-3034-02 | 0.25grams | .25grams | 4 | .07 | N/A | 12.13 | 11.39 | ||
| 10-3034-05 | 0.5grams | .5grams | 8 | .07 | N/A | 25.03 | 23.52 | ||
| 10-3034-10 | 1.0gram | 1grams | 15.99 | .07 | N/A | 50.81 | 47.73 | ||
| 10-3034-2S | 20grams | 20grams | 319.89 | .07 | N/A | 1031.13 | 968.64 | ||
08/15/2012 | http://www.glenres.com/ProductFiles/10-3034.html