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Catalog Number: 10-1085-xx
Description: 2-Amino-dA-CE Phosphoramidite
| 5'-Dimethoxytrityl-N2,N6-bis(diisobutylaminomethylidene)-2,6-diamino- 2'-deoxypurine riboside-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C58H83N10O6P | M.W.: 1047.33 | F.W.: 328.22 |
Diluent: Anhydrous Acetonitrile |
| Coupling: Standard coupling time. Add a maximum of 3 times when using 0.02 M I2 oxidation or use 0.5 M CSO in anhydrous ACN (40-4632-xx) and a 3 minute oxidation time for best results with multiple additions. |
| Deprotection: Ammonium hydroxide 24h/55°C; AMA 1.5h/65°C or 17h/room temperature. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 2-3 days |
| Catalog Information | Material Safety Data Sheet |
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1085 | 2,6-diaminoPurine | 278 | 10.2 | 255 | 9.3 | 8.5 |
Literature Highlights
| Glen Report 11.1: USING MODIFIED BASES TO OPTIMIZE HYBRIDIZATION |
| Glen Report 23.2: Technical Brief - Improved oligo synthesis using 2-Amino-dA and CSO Oxidation |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1085-02 | 0.25grams | .25grams | 2.39 | 66.33 | 39.8 | 24.88 | 18.09 | 13.27 | 3.32 |
| 10-1085-90 | 100µmoles | .105grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1085-95 | 50µmoles | .052grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1085-02 | 0.25grams | .25grams | 3.56 | .07 | 64.8 | 40.5 | 29.45 | 4.05 | |
| 10-1085-90 | 100µmoles | .105grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1085-95 | 50µmoles | .052grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1085-02 | 0.25grams | .25grams | 3.56 | .07 | 66.4 | 41.5 | 30.18 | ||
| 10-1085-90 | 100µmoles | .105grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1085-95 | 50µmoles | .052grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
08/15/2012 | http://www.glenres.com/ProductFiles/10-1085.html