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ALTERNATIVE SOLVENTS/REAGENTS

Glen Research offers alternative solvents and reagents in suitable bottles and formulations for use on various DNA synthesizers. All solvents and reagents are prepared to our exacting specifications to ensure the highest coupling efficiencies and are passed through a 0.2 micron filter during packaging to eliminate particulate contamination. Glen Research offers the activators below in powder form for later dissolution in anhydrous acetonitrile or as a prepared solution.
Item Catalog No. Pack Price ($)
Activator
5-Ethylthio-1H-tetrazole (ETT) 30-3040-10 1g 35.00
(Dissolve 1g in 31mL anhydrous acetonitrile 30-3040-20 2g 60.00
for a 0.25M solution) 30-3040-25 25g 500.00
0.25M 5-Ethylthio-1H-tetrazole in Acetonitrile
(Applied Biosystems) 30-3140-45 45mL 40.00
  30-3140-52 200mL 100.00
  30-3140-57 450mL 200.00
  30-3140-62 2L 760.00
  30-3142-52 200mL 100.00
  30-3140-57 450mL 200.00
4,5-Dicyanoimidazole (DCI), crystalline 30-3050-10 1g 35.00
(Dissolve 1g in 34mL anhydrous acetonitrile 30-3050-25 25g 500.00
for a 0.25M solution)      
A license may be required from Isis Pharmaceuticals, Inc. to use DCI as an activator in oligonucleotide synthesis as claimed in U.S. Patent Numbers 5,734,041; 6,031,092; 6,160,109 and foreign equivalents.
0.25M DCI in Acetonitrile
(Applied Biosystems) 30-3150-45 45mL 40.00
  30-3150-52 200mL 100.00
  30-3150-57 450mL 200.00
  30-3150-62 2L 760.00
(Expedite) 30-3152-52 200mL 100.00
  30-3150-57 450mL 200.00
5-Benzylthio-1H-tetrazole 30-3070-10 1g 35.00
(Dissolve 1g in 21.3mL anhydrous acetonitrile 30-3070-20 2g 60.00
for a 0.25M solution) 30-3070-25 25g 500.00
5-Benzylthio-1H-tetrazole in Acetonitrile
(Applied Biosystems) 30-3170-45 45mL 40.00
  30-3170-52 200mL 100.00
  30-3170-57 450mL 200.00
  30-3170-62 2L 760.00
(Expedite) 30-3172-52 200mL 100.00
  30-3170-57 450mL 200.00
Saccharin 1-Methylimidazole (SMI) 30-3080-10 1g 35.00
(Dissolve 1g in 18.8mL anhydrous 30-3080-20 2g 60.00
acetonitrile for a 0.2M solution) 30-3080-25 25g 500.00
0.2M Saccharin 1-Methylimidazole (SMI) in Acetonitrile
(Applied Biosystems) 30-3180-45 45mL 40.00
  30-3180-52 200mL 100.00
  30-3180-57 450mL 200.00
  30-3180-62 2L 760.00
(Expedite) 30-3182-52 200mL 100.00
  30-3180-57 450mL 200.00
Cap Mix A
THF/Lutidine/Ac2O 40-4010-52 200mL 30.00
  40-4010-57 450mL 72.00
  40-4010-62 2L 325.00
THF/Ac2O (9:1) 40-4012-62 2L 275.00
Cap Mix B
6.5% DMAP in THF
(Cap B solutions containing DMAP are preferred by some researchers for preparing long oligos.)		 40-4020-52 200mL 42.00
10% MeIm in THF
(This Cap B solution is identical to the formulation produced by Applied Biosystems.)		 40-4120-52 200mL 30.00
40-4120-57 450mL 72.00
  40-4120-62 2L 325.00
10% MeIm in THF/Pyridine (8:1) 40-4122-62 2L 325.00
Oxidizing Solution
0.02M I2 in THF/Pyridine/H2O 40-4132-62 2L 325.00
0.5M CSO in Anhydrous Acetonitrile (ABI)40-4632-52 200mL250.00
0.5M CSO in Anhydrous Acetonitrile (Expedite)40-4632-52E 200mL250.00
   (CSO is an alternative anhydrous Oxidizer. Please see a more complete description on page 36)
Deblocking Mix
3% DCA/DCM
(DCA solutions are more mildly acidic than the TCA equivalents, possibly causing less depurination of dA sites.)		 40-4040-57 450mL 36.00
40-4040-62 2L 144.00
2.5% DCA/DCM 40-4042-57 450mL 36.00
40-4042-62 2L 144.00
DCA 40-4044-54 120mL 60.00
(Add to 4L of DCM to make a 3% solution.)      

ABBREVIATIONS

Ac2O = Acetic Anhydride
DCA = Dichloroacetic Acid
DCM = Dichloromethane
DMAP = Dimethylaminopyridine
I2 = Iodine
MeIm = 1-Methylimidazole
TCA = Trichloroacetic Acid
THF = Tetrahydrofuran

 

5-Ethylthio-1H-tetrazole

DCI

5-Benzylthio-1H-tetrazole

 

 

 

 

 

 

 

 

 

INTELLECTUAL PROPERTY

SMI is sold under license from Avecia Biotechnology, Inc.

 

 

OTHER INSTRUMENT TYPES

All minor bases, RNA products and modifiers are packaged in septum-capped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.

Monomers

Columns
For Instrument type
Add
Add
Applied Biosystems 3900
  
A
Expedite
E
E
Beckman Oligo 1000
B
  
Pharmacia Gene Assembler
P
 
Mermade
M
M

Please inquire for availability
of columns for other
instrument types.

INTELLECTUAL PROPERTY

This capping reagent is supplied under license.

 

 

UniCap Phosphoramidite

Alternative Capping Reagent

The phosphoramidite of diethylene glycol monoethyl ether, UniCap, is the basis for an alternative capping reagent. To use UniCap as a capping amidite on the Expedite 8909 or AB synthesizers, dilute it to the standard amidite concentration and place the vial in position 5 on the instrument. Cycles can be modified by adding coupling steps for amidite reservoir 5 after the last column coupling step. The standard capping steps can be left out of the cycle. UniCap Phosphoramidite was originally developed for oligo synthesis on the surface of chips and is the capping reagent of choice for this application.
Item Catalog No. Pack Price ($)
UniCap
UniCap Phosphoramidite 10-4410-02 0.25g 50.00
  10-4410-05 0.5g 100.00
  10-4410-10 1.0g 200.00
  10-4410-20 2.0g 400.00

http://www.glenres.com/Catalog/reagents.html

 		 
Please contact Glen Research if you have any questions or comments!
 
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