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PHYSICAL DATA

The physical data table contains information which is unique to each monomer phosphoramidite. The molecular weight (MW) is the formula weight of the fully-protected monomer phosphoramidite. The MW is used to calculate the volume of solvent required to dilute 0.25g of the monomer to give a final 0.1M concentration. This figure is also shown in the table. The unit molecular weight (Unit FW) is the formula weight of each monomer once inserted into an oligonucleotide with all protecting groups removed. To obtain the molecular weight of a specific oligonucleotide, the following formula is used:

Oligonucleotide MW = Sum of Unit FW - 61.96 
Cat. No.
Item
Phosphoramidite MW Unit FW Dilution (0.1M)
20-2001
dA-CPG 1000
313.21
20-2002
dA-CPG 2000
313.21
20-2004
3’-dA-CPG
313.21
20-2010
dC-CPG 500
289.18
20-2011
dC-CPG 1000
289.18
20-2012
dC-CPG 2000
289.18
20-2013
Ac-dC-CPG 500
289.18
20-2015
Ac-dC-CPG 1000
289.18
20-2017
2’,3’-ddC-CPG
273.19
20-2019
3’-Amino-Modifier C6 dC CPG
457.42
20-2020
dG-CPG 500
329.21
20-2021
dG-CPG 1000
329.21
20-2022
dG-CPG 2000
329.21
20-2029
dmf-dG-CPG
329.21
20-2030
dT-CPG 500
304.20
20-2031
dT-CPG 1000
304.20
20-2032
dT-CPG 2000
304.20
20-2039
3’-Amino-Modifier C6 dT CPG
458.41
20-2040
dI-CPG 500
314.19
20-2041
dI-CPG 1000
314.19
20-2050
dU-CPG 500
290.17
20-2051
dU-CPG 1000
290.17
20-2056
3’-Fluorescein-dT CPG
815.71
20-2060
5-Me-dC-CPG
303.21
20-2064
3’-dC-CPG
289.18
20-2074
3’-dG-CPG
329.21
20-2084
3’-dT-CPG
304.20
20-2090
5-Br-dU-CPG
369.07
20-2601
Pac-dA-CPG
313.21
20-2621
iPr-Pac-dG-CPG
329.21
20-2900
3’-Phosphate CPG
79.98
20-2902
3’-Glyceryl CPG
154.06
20-2903
3’-CPR II CPG
79.98
20-2913
3’-Spacer C3 CPG
138.06
20-2933
3’-Thiol-Modifier C3 S-S CPG
154.12 (thiol) 244.27 (disulfide)
20-2937
3’-DTPA CPG
214.19
20-2938
3’-Thiol-Modifier 6 S-S CPG
198.18 (thiol) 332.37 (disulfide)
20-2952
DesthiobiotinTEG-CPG
539.56
20-2954
3’-PT-Amino-Modifier C3 CPG
137.07
20-2955
3’-BiotinTEG CPG
569.61
20-2956
3’-PT-Amino-Modifier C6 CPG
179.15
20-2957
3’-Amino-Modifier C7 CPG 500
209.18
20-2958
3’-Amino-Modifier C7 CPG 1000
209.18
20-2961
3’-(6-FAM) CPG
569.46
20-2963
3’-Fluorescein CPG
598.56
20-2964
3’-(6-Fluorescein) CPG
566.48
20-2973
3’-Acridine CPG
450.86
20-2975
3’-Cholesteryl-TEG CPG
755.97
20-2980
3’-Uaq Cap CPG
539.39
20-2992
3’-Alkyne-Modifier Serinol CPG
334.26
20-2993
3’-Protected Biotin Serinol CPG
450.45
20-2994
3’-6-Fluorescein Serinol CPG
584.47

 
Please contact Glen Research if you have any questions or comments!
 
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